FastQCFastQC Report
Sun 14 Apr 2019
SRR6575797_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575797_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1485329
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA31070.2091792458101875No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC25320.17046728367923875No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC20300.13667005761013215No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15640.10529653699618065No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15270.10280550638949351No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15120.10179562911651224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22650.049.9310461
TCAACGC29100.037.469744
ATCAACG29800.036.905013
CAACGCA30100.036.2249035
GGTATCA10450.036.074581
AACGCAG31100.034.9089936
GTACATG57800.030.9801371
TATCAAC35850.030.8080652
TACATGG58350.030.6075422
AGGTATA6500.029.6453276
TTAGGTA6400.029.3741784
ACATGGG61400.028.85753
TAGGTAT6900.028.6078955
ACGCAGA38150.028.3337737
GTCTTAG8350.028.2170181
CTTAGGT7300.026.3965233
ATTATCC11800.026.28743
CATGGGG36900.026.1103824
CGCAGAG41650.025.9527828
TATTATC12800.024.2336962
Produced by FastQC (version 0.11.2)