FastQCFastQC Report
Sun 14 Apr 2019
SRR6575798_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575798_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1343638
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA28080.20898486050558263No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC24130.179587061396001No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC21140.15733404384216582No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15750.1172190723989646No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15110.11245588469513366No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14220.10583207679449376No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13770.10248296044023764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22300.047.0079461
TAAGTCG500.001703828537.5923655
ATCAACG27550.036.502113
TCAACGC27550.036.502114
CAACGCA28250.035.7626465
GGTATCA9400.035.0059171
AACGCAG29250.034.700646
GTACTAA959.4397546E-734.6374361
GTCTTAG9050.033.7626151
TAGGTAT6900.033.3700335
CTTAGGT7250.032.4084173
TTAGGTA7350.031.3281354
GTACATG53800.031.0182571
AGGTATA7450.030.9064736
TACATGG55050.030.0599982
TCTTAGG9400.030.0073072
TATCAAC34600.029.6199282
ACATGGG56700.028.8417763
GGTATAG8150.028.2519287
TATAGTA8550.027.477759
Produced by FastQC (version 0.11.2)