FastQCFastQC Report
Sun 14 Apr 2019
SRR6575801_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575801_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1395884
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC19400.13898002985921468No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA19070.13661593656779505No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18570.13303397703534103No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA18000.12895054316834348No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17500.1253685836358895No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17110.12257465520057541No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16710.11970908757461221No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16460.11791810780838521No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16430.117703190236438No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15980.11447942665722939No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15270.10939304412114473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19300.048.227141
GGTATCA8450.039.498921
TCAACGC24450.037.676234
ATCAACG24400.037.5608183
CAACGCA25550.036.054165
AACGCAG26700.034.325246
GTACATG52450.029.845741
TACATGG52450.029.3079782
ACATGGG55550.027.412663
ACGCAGA34100.026.8763627
TATCAAC34300.026.8624442
GTATAGG2357.2759576E-1226.0051331
GTCTTAG9250.025.9186291
CTTAGGT6900.025.2024573
CGCAGAG37500.024.8155638
TAGTATC1151.3775784E-424.521317
AGGTATA6300.023.8725996
TAGGTAT6550.023.6789765
CTAGTAC1602.0396365E-623.4995883
TATAGTA7300.023.1776759
Produced by FastQC (version 0.11.2)