FastQCFastQC Report
Sun 14 Apr 2019
SRR6575802_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575802_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1231845
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA18460.14985651603894973No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18000.1461222799946422No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17290.14035856783929795No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17120.138978524083793No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17010.13808555459493688No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16380.13297127479512438No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA15260.12387922181767998No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14860.12063206004002128No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14640.11884612106230898No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14160.11494952692911853No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13590.11032232139595484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18050.051.297511
TCAACGC22050.040.4898764
ATCAACG22050.040.276773
CAACGCA23400.037.9531145
AACGCAG24100.036.850746
GGTATCA7300.034.1240231
CTTAGGT5550.033.8663563
TAGGTAT5450.032.763375
GTTCTAG2250.031.3340071
AGGTATA5750.031.0539766
GTACATG46500.030.6264691
TATCAAC29350.030.4290242
TACATGG47100.029.9393832
ACGCAGA29900.029.7024357
ACCTAAG7600.029.684851
ACATGGG48300.029.1860683
TTAGGTA6350.028.8597354
GTCTTAG7300.027.685531
CTAAGAC10400.027.1093673
CGCAGAG32950.026.8104368