FastQCFastQC Report
Sun 14 Apr 2019
SRR6575802_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575802_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1231845
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA20280.16463110212729684No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17750.14409280388360549No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16330.13256537957291706No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC16290.13224066339515117No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14640.11884612106230898No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13580.11024114235151337No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13450.1091858147737743No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT13130.10658808535164732No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13000.10553275777390825No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12760.10358446070731302No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT12590.10220441695180806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16850.049.458161
ATCAACG21550.037.9450263
TCAACGC21600.037.8571854
CTTAGGT5100.037.7803963
TTAGGTA5350.036.8933754
GTCTTAG6850.036.4292221
CAACGCA22450.036.423845
GGTATCA7800.035.61411
AACGCAG23300.035.0922286
AGGTATA5650.034.099896
TAGGTAT5900.032.657635
GTACATG45900.030.1577721
GGTATAG6400.030.1050327
TACATGG46100.029.5642972
GTATAGT6900.029.3070458
ACCTAAG7550.028.6863631
TCTTAGG8250.028.4830532
ACATGGG47450.028.325833
TATAGTA7200.028.0859189
TATCAAC29950.027.6176262