FastQCFastQC Report
Sun 14 Apr 2019
SRR6575803_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575803_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590521
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12500.21167748479732304No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7600.1286999107567724No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA7300.12361965112163666No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7150.12107952130406877No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6900.11684597160812231No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6770.11464452576623015No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5920.1002504568000122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12900.044.1452871
TACATGG32850.035.6227152
GGTATCA7150.035.5448271
GTACATG32850.035.5308041
ACATGGG32950.034.9440883
ATGGGTA5650.030.7762955
TCAACGC17850.030.5409684
ATCAACG18050.030.462933
CAACGCA18250.029.8715785
AACGCAG18900.029.090446
GGGTACC5650.028.2785247
TACACTA1354.6315108E-727.8496195
CATGGGA12700.027.753684
GGTACCT5550.027.11078
CATGGGG16700.027.0157974
ATGGGAG6500.026.75175
ATTATAC1956.039045E-1026.5106933
CATGGGT10300.026.0076264
GTTTAAT1659.179894E-825.6712631
TATCAAC22250.024.9238432
Produced by FastQC (version 0.11.2)