FastQCFastQC Report
Sun 14 Apr 2019
SRR6575805_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575805_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016758
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA16230.15962500417995235No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC12110.11910405425873216No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11300.1111375568227641No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC10950.10769524311586434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16650.057.31021
TCAACGC21350.045.1187024
ATCAACG21900.043.985583
CAACGCA22300.043.405185
AACGCAG23100.041.9019786
GGTATCA6250.039.1087041
TATCAAC26000.037.2429432
TATAGGG1107.6866854E-834.1859322
ACGCAGA29100.033.423867
CGCAGAG31750.030.6341538
GTACATG42750.030.2375561
TACATGG43000.030.0617582
ACATGGG43850.029.2545663
GTCTTAG5050.028.8549581
GCAGAGT34550.028.0155039
CTAAGAC7050.027.3272113
TAGGTAT4150.027.1733025
ACCTAAG5800.026.7445981
AGGTATA4050.026.6840696
TTAGGTA4350.025.9252284
Produced by FastQC (version 0.11.2)