FastQCFastQC Report
Sun 14 Apr 2019
SRR6575805_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575805_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016758
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA16540.1626739106060636No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC12120.11920240607892929No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC11620.11428481506907248No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10490.10317105938679608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17100.053.9574281
TCAACGC21750.041.4844254
ATCAACG22150.040.7352683
CAACGCA22350.040.3707475
GGTATCA6550.039.5287131
AACGCAG23450.038.4770246
TATCAAC26500.034.7595862
GTACATG41800.030.745221
ACGCAGA29200.030.7392737
TACATGG43100.029.6589742
GTCTTAG5500.029.100971
ACATGGG43700.029.035243
CGCAGAG32550.027.4488038
AGGTATA3800.027.2070966
CTTAGGT3900.026.5094783
CATGGGG25550.026.4858974
GTATAGA1257.923858E-626.3620571
GAACGGT909.5239276E-426.1078176
GCAGAGT34550.025.9959779
TAGGTAT4200.024.6159445
Produced by FastQC (version 0.11.2)