FastQCFastQC Report
Sun 14 Apr 2019
SRR6575806_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575806_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855130
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22200.2596096499947376No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT18500.2163413749956147No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC16680.19505806134739748No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC15070.17623051465858994No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13890.16243144317238314No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG13810.16149591290213183No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA13120.15342696432121433No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT12250.14325307263223136No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11990.1402125992539146No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA11680.13658741945669078No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11420.13354694607837406No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11400.13331306351081124No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11020.1288692947271175No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC10010.11705822506519477No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9810.11471939938956649No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC9430.1102756306058728No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9410.11004174803830996No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9050.10583186182217907No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA8870.10372691871411366No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG8830.10325915357898799No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8670.10138809303848538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTACC3500.034.9134147
TAGTACT5950.031.5958524
CAGTCCG600.004160898731.33255494
ATGGGTA4400.030.9765035
TGGGTAC4300.029.5108936
GTATAAC804.7756586E-429.3794251
GTCTAGA650.006148774628.9274311
TACCGAT650.00615404728.9223587
CATGGGT6300.028.3485014
CATGGGG22000.028.1992994
ATGGGGG14850.027.2181785
CTGTGCG7300.027.0404229
GGTACCT4550.026.8564748
CCTAGTA7400.026.6796912
ACTGTGC8050.026.2726388
CTAGTAC7550.026.1450443
TAGGTAT909.517061E-426.110465
GTTTAAT1458.634779E-725.9349381
CTATTGA3650.025.7527839
GTACTGT7350.025.5775956
Produced by FastQC (version 0.11.2)