FastQCFastQC Report
Sun 14 Apr 2019
SRR6575806_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575806_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855130
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20080.23481809783307803No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT18490.2162244337118333No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC15710.1837147568206004No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC14010.16383473857776013No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA12780.14945096067264627No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11840.1384584799971934No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11580.13541800661887668No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA11470.1341316524972811No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11430.13366388736215545No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG11330.13249447452434132No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC10870.1271151754703963No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10610.12407470209207956No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9950.11635657736250628No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9780.11436857553822227No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9380.10969092418696572No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC9020.10548103797083486No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC8810.10302527101142517No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8740.10220668202495525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAT804.640242E-946.9971055
CTTAGGT954.274625E-1044.5235753
TTAGGTA1256.2664185E-933.8379144
AGGTATA1201.6523518E-731.3314026
GTACTAG4700.031.0999281
TAGTACT7150.029.5785984
GGGTACC3100.028.804687
CATGGGT6050.028.7420314
CATGGGG22950.028.464484
GTACTGT7700.028.0761936
CTAGTAC8100.027.8501383
CCTAGTA8300.027.1790492
ATGGGGG13750.026.6601755
ATGGGTG2500.026.3183785
TAGGCAT8100.025.5292915
GTATAAG5750.025.4208091
ATGGGTA4450.025.3467545
TACTGTG8900.025.3467547
ACTGTGC9550.025.0979298
ATACTTA950.001304780724.735326
Produced by FastQC (version 0.11.2)