FastQCFastQC Report
Sun 14 Apr 2019
SRR6575807_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575807_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803258
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA24070.2996546564117631No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20670.2573270356473263No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC14550.18113731827133997No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC13830.17217382210945922No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12260.1526284207564693No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG12210.15200595574522754No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA11780.1466527566485488No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11310.14080158554287664No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11080.13793824649116473No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA10950.13631983746193627No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10790.13432794942596277No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10600.13196258238324424No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10170.12660938328656546No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9420.1172724081179397No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC9420.1172724081179397No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT8910.11092326500327418No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC8630.10743746094032056No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT8610.10718847493582385No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG8510.10594354491334042No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT8460.10532107990209871No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8300.10332919186612521No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8210.10220875484589012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTATA652.789675E-643.3714836
GTACTAG3800.038.3495981
GGGTACC3250.033.2514727
TAGGTAT851.7577631E-533.166435
CTTAGGT851.7577631E-533.166433
TAATAAT3550.033.0885733
TTAGGTA1052.0692223E-631.323854
TAGGCAT6600.031.3238495
TGGGTAC3650.030.8947566
CTAGGCA6650.030.381784
ATGGGTA4500.028.1914675
GGTACCT4050.027.8434248
GTTAATA4650.027.2956561
TAACCAC1555.0944436E-827.2820645
CTATTGA3800.027.2022919
TCTTAGG1406.353457E-726.8623922
AGGGCTA4100.026.3578765
GACGTTA1258.0388545E-626.3120337
TCAAGAC1851.0059011E-825.3977173
CTAACTG2251.1641532E-1025.0590824
Produced by FastQC (version 0.11.2)