FastQCFastQC Report
Sun 14 Apr 2019
SRR6575807_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575807_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803258
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21850.27201720991263084No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20950.2608128397102799No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC14890.18537008034778366No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC13900.17304527312519763No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA12160.15138349073398583No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA11340.14117506454962167No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC11250.14005462752938658No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11110.13831172549790977No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11060.13768926048666805No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG10780.13420345642371442No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9940.12374604423485355No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT9870.12287459321911516No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9670.12038473317414829No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9420.1172724081179397No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9330.11615197109770461No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC8400.10457412188860864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC3250.033.2565163
TCTAACG3450.031.3305512
TAGTACT5700.031.32864
AGGGCTA4450.030.624595
CTATTGA4400.029.9250649
ACCTAAG2051.8189894E-1229.8561331
ACACTCG953.7372396E-529.7000648
ACGCCTA3900.028.918716
CTAGTTG650.006157620328.9187095
CTAGTAC6850.028.127143
AACGCCT4200.027.9719685
GTCCTAC3900.027.7656141
TATATGC856.820306E-427.6428857
ATATAGT3600.027.4125274
GTACTGT6900.027.2422646
CCTAGTA7300.027.0386962
TACACTA4000.027.020925
ACGTACA700.008838288526.854762
TAGGTAT700.00884097326.8530885
CACTATC3850.026.8530887
Produced by FastQC (version 0.11.2)