FastQCFastQC Report
Sun 14 Apr 2019
SRR6575809_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575809_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882525
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13870.1571626866094445No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12270.1390328885867256No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12260.1389195773490836No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11900.13484037279397185No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG11040.12509560635676045No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10440.11829693209824085No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10120.11467097249369705No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9480.1074190532846095No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG9460.1071924308093255No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9200.10424633863063369No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT9100.10311322625421376No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8860.10039375655080593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15050.047.2144931
GGTATCA6950.041.979941
ATCAACG20700.033.8248023
TCAACGC20650.033.679144
CAACGCA21750.032.191885
AACGCAG22800.031.1233276
GTACATG40100.030.1594661
TACATGG40700.029.6727682
ACATGGG41700.028.735813
GTATATA1503.6516212E-828.2348961
GGTACCT5500.026.5041398
ACGCAGA26950.026.1563077
TATCAAC27800.025.3551252
CATGGGG21500.024.6978594
CGCAGAG29900.023.590378
GGGTACC6500.023.1355887
ATTATCC4400.022.4277633
GCAGAGT32300.022.1286939
AGAGTAC28050.021.8772310-11
GTCTTAG3301.8189894E-1221.390071
Produced by FastQC (version 0.11.2)