FastQCFastQC Report
Sun 14 Apr 2019
SRR6575810_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575810_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918911
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11780.12819522238824No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11400.12405989263378064No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC11210.12199222775655097No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10050.10936858955872766No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9600.10447148853371001No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA9570.10414501513204216No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9500.10338324386148386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14300.049.633681
GGTATCA6100.037.7573781
ATCAACG19050.036.2669223
TCAACGC19100.036.171984
CAACGCA19300.036.0406575
AACGCAG20800.033.8934826
GTACATG36650.030.2672961
TACATGG36800.029.8884662
ACATGGG38200.028.666913
ACGCAGA24750.028.2943277
TATCAAC24850.028.1835352
TAAGACA6150.025.2189564
CATGGGG23900.025.1709944
CGCAGAG28100.024.5866478
GTATAGG1751.6440572E-724.1735271
AACCGTG2151.877197E-924.0459847
GCAGAGT29800.023.6571969
GTACTAC1802.1581218E-723.502041
CTAAGAC6050.023.3052733
AGAGTAC25450.022.7146310-11
Produced by FastQC (version 0.11.2)