FastQCFastQC Report
Sun 14 Apr 2019
SRR6575815_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575815_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216039
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6270.2902253759737825No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3760.17404264970676592No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3600.166636579506478No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3200.14812140400575824No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2940.13608653993029035No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2810.13006910789255643No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2730.1263660727924125No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2600.12034864075467855No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2540.11757136442957059No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2380.11016529422928267No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2250.10414786219154874No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2170.10044482709140479No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC250.00523065856.3990333
GTATATG405.666908E-446.999191
ATATACT708.5665306E-846.9991874
GTATCAA5950.041.8648261
GTACATG11800.040.228121
TACATGG12150.038.682462
ACATGGG12750.036.493493
TATTATG651.4098366E-436.1532252
ATACACT651.4098366E-436.1532254
TCAACGC7100.035.08394
AACGCAG7150.034.8385626
GGGTACC2300.034.7385337
ATCAACG7450.034.066533
TGGGTAC2350.033.9994166
CATGGGG7350.033.890574
CAACGCA7350.033.890575
CATGGGT3500.032.2280164
GGTACCT2500.031.9594488
ATGGGTA2650.031.9239795
TATATAC600.004153923631.3327923
Produced by FastQC (version 0.11.2)