FastQCFastQC Report
Sun 14 Apr 2019
SRR6575815_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575815_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216039
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5120.23699424640921313No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3460.16015626808122607No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3220.14904716278079422No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3000.13886381625539831No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2980.13793805748036234No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2930.13562366054277236No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2790.12914334911752046No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2760.12775471095496646No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2630.12173727891723253No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2460.11386832932942664No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2340.10831377667921069No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2280.10553650035410272No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2230.10322210341651276No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2190.10137058586644078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA5850.044.2978941
AACCGAT250.001701407337.597532-33
TAACAGT550.002713033534.1795466
AACGCAG7650.033.7886056
GTACATG11250.033.08651
TACATGG11250.031.7492
ACGTAGG600.00414551531.34576294
CAACGCA8200.030.9491625
TCAACGC8250.030.7615914
GGTATCA3450.030.0455271
ACATGGG11800.029.8708973
ATCAACG8850.028.6760583
CAAACCG350.00883289126.8553630-31
TTAGAGT700.00882329126.855364
TACTGAT700.00882329126.855367
ACGCAGA9400.026.4982387
TATCAAC9900.026.1093752
TATTGGA2900.025.929312
CGCAGAG9700.025.6787058
CATGGGT3700.025.4037154
Produced by FastQC (version 0.11.2)