FastQCFastQC Report
Sun 14 Apr 2019
SRR6575816_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575816_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672352
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA13690.20361358336109656No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12970.19290490695350054No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC10860.16152253581457332No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC9280.13802294036457094No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9180.13653562419684925No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT9140.13594069772976058No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA7980.11868783018418924No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG7880.11720051401646757No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT7600.11303602874684689No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG7310.10872281186045404No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7210.10723549569273239No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA7140.10619437437532722No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT6800.10113749940507354No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT6760.10054257293798487No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6750.1003938413212127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTGA1850.033.0258759
GTATATA902.5355137E-531.4351041
GTATAAC753.2124275E-431.4351021
GTACTAG3650.031.0044841
AGGGCTA2300.030.6511065
TAGTACT4050.029.0113374
GTCTATA856.68517E-427.7368551
TAATCCA1205.840033E-627.4157124
GTATAAG3800.027.2989031
ATAAGGT3650.027.0401553
TAACCAC1057.418827E-526.8562095
CTGTGCG4900.026.8562099
GTACTGT4750.026.7148596
ACCTAAG909.335155E-426.1959191
CATGGGG16950.025.232164
GTATCAA27200.024.4431021
CTCTTAC1751.5933256E-724.2499371
ACTGTGC5750.023.7035228
CTAGTAC5000.023.4991823
GGCTATT3000.023.4991827
Produced by FastQC (version 0.11.2)