FastQCFastQC Report
Sun 14 Apr 2019
SRR6575820_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575820_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085944
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17870.1645572884052953No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA15660.14420633108152908No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15070.13877327007654172No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC15050.1385890985170506No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14800.13628695402341187No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14520.13370855219053654No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14410.13269560861333551No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG14240.13113015035766118No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14090.12974886366147795No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT12810.11796188385404772No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12380.11400219532498913No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11540.10626698982636307No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA11480.10571447514788976No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11470.10562238936814422No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG11190.10304398753526885No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC11140.10258355863654112No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11110.10230730129730448No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA11050.10175478661883118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG3900.042.257011
CTTAGGT2150.030.6030163
TTAGGTA1802.382876E-1028.7206884
GTATCAA32100.027.4303841
TAGTACT8150.026.5261924
ACCTAAG2850.026.434461
CCTAGTA8500.024.8810232
GGGTACC6050.024.8581777
CATGGGG27850.024.6378234
CTGTGCG9550.024.6060169
GTCTATA1551.5221049E-624.302651
GTACATG56000.024.2999361
TCTTAGG3100.024.256772
ACATGGG55300.024.1361433
TACATGG56150.023.9381712
CTAGTAC8700.023.7688473
CAACGCA35800.023.236195
CCTAAGA3850.023.1935672
ATCAACG36250.022.9477393
TACTAGG7600.022.8803582
Produced by FastQC (version 0.11.2)