FastQCFastQC Report
Sun 14 Apr 2019
SRR6575821_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575821_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007484
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA16520.1639728273600375No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT16450.16327802724410512No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15890.15771962631664624No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14480.14372436683858006No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14180.1407466520560128No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC14040.13935705182414806No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13860.13757042295460772No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13600.1349897368097161No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG12970.12873653576632482No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11950.11861230550559612No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA11680.11593236220128558No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT11350.11265687594046159No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11290.11206133298394813No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA11080.10997693263615105No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG10820.10739624649125942No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG10660.10580813194055688No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10620.10541110330288123No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA10590.1051133318246245No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC10380.10302893147682742No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT10340.10263190283915177No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT10110.10034898817251688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG3150.041.801681
ATGGGTA6750.028.5432175
GGGTACC6150.027.5074427
TGGGTAC6700.027.3534876
TAGGCAT5800.026.7367615
TAGTACT5150.026.4614494
GTATCAA33700.025.536861
GTATAAG4450.025.3628181
CATGGGT9350.025.1293494
TAGGACT3750.025.062344
CTAGGCA6850.024.6964664
CATGGGG25750.024.6365224
GTAGGAC3950.023.793363
TATTAGG4150.023.7790263
CTATAAG2202.4338078E-923.5134491
TAGGAGT1401.9335908E-523.4959434
TGTAGGA5100.023.036382
CTATTAG4300.022.9506652
CTATTGA2704.5474735E-1122.6414579
GGTACCT7300.022.546028
Produced by FastQC (version 0.11.2)