FastQCFastQC Report
Sun 14 Apr 2019
SRR6575823_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575823_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855548
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA19000.22207988330286552No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT18160.21226161477789673No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC16270.19017051059671697No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC14690.1717028150378471No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG13760.16083258917091736No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA13570.1586117903378887No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12710.14855975351470635No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA12640.14774156447095896No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11170.13055959455226357No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11080.12950763721030265No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10180.11898806379069321No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9830.11489711857195622No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC9810.11466335027374268No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9610.11232566729160724No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT9570.11185813069518016No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC9410.10998798430947182No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA9330.10905291111661765No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9100.10636457568716191No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT9000.10519573419609421No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG8560.10005283163539627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATAAT3200.035.2400743
TATTAAG1107.687777E-834.1841772
ACCTAAG1405.0931703E-1033.5737461
CATGGGT6450.030.5960314
GTATATA1102.9656785E-629.9111561
GTGCGTA650.00616177328.9149394
GGGTACC4300.028.4105997
TAGGTAT1005.3321895E-528.1920575
AACCGTG1853.2923708E-1027.9380747
GTACTAG4300.027.3274691
ACGCTAA350.008849353526.84957992-93
TAGTATG700.00884692526.8495797
ATGGGTA4650.026.2721675
CATGGGG20400.025.7966564
TAGTACT6400.025.6958874
ATGGGGG13700.025.3797115
GTACTGT6950.025.0145346
TGGGTAC4700.024.9929566
GAACCGT1901.3358658E-824.7298766
GCTACCG950.001306455824.7298769
Produced by FastQC (version 0.11.2)