FastQCFastQC Report
Sun 14 Apr 2019
SRR6575823_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575823_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855548
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA18520.21646944414574049No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT16940.19800174858687064No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC15500.18117043111549558No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC13650.15954686353074288No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA13190.15417019267183138No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA12510.1462220705325709No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG11910.13920902158616466No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11530.13476742392010735No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC11150.13032582625405004No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11010.12868944816655523No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10860.12693618592995365No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9730.1137282770808885No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9540.11150747824785986No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9340.10916979526572443No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8640.10098790482825043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATG2200.029.9498231
CATGGGG21150.028.43994
GAACCGT1354.6388413E-727.8474036
TAGTACT6250.027.8195534
GTATAAG5200.027.1523111
ATGGGGG13950.026.9490995
CTTTATA700.00883662826.8559912
CGTTAAC1606.8210284E-826.4735031
CTACGCT909.5245615E-426.1069374
ACCTAAG1458.544066E-725.9663491
GTATATA1101.00866404E-425.6712761
TGGGGGG8100.025.5267856
CTAGTAC8050.025.101583
GAGTAGT2651.8189894E-1224.8640041
AATCGAC950.00130552824.7328916
CCTAGTA8300.024.3483542
TAATGGT1751.6476952E-724.1675664
CTGTGCG6650.024.0445049
ATAAGGT5300.023.939573
TAGTAGT3150.023.8692024
Produced by FastQC (version 0.11.2)