FastQCFastQC Report
Sun 14 Apr 2019
SRR6575824_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575824_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310808
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA27090.20666642254243184No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC24950.19034061433863692No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC18410.14044772384666557No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14810.11298374742906665No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14680.11199199272509781No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14590.11130539331465783No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14090.10749095214554687No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13740.10482084332716919No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20350.055.6701051
TCAACGC25950.044.1915324
ATCAACG25850.043.8170473
CAACGCA26600.042.9349755
GGTATCA6800.042.861
AACGCAG27500.041.7007336
TATCAAC32650.035.4177782
ACGCAGA34250.033.345127
GTACATG52000.032.4534231
TACATGG53200.031.2795892
ACCTAAG11700.030.5349461
ACATGGG55300.030.1710073
CGCAGAG37900.030.0097758
TAAGACA14150.029.2289244
ACGACCG650.00615526128.9223275
CTAAGAC13650.028.9223253
ACCAGAT12500.028.19926694
GCAGAGT41900.027.2570469
CATGGGG35800.026.1250214
CCTAAGA14600.026.079642
Produced by FastQC (version 0.11.2)