FastQCFastQC Report
Sun 14 Apr 2019
SRR6575824_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575824_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310808
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA29680.22642522779842664No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC24620.1878230831670237No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC19270.14700856265753642No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14960.11412807977979995No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14670.11191570390171558No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14070.10733837449878243No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG13120.10009093627747159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19800.052.324511
GGTATCA7300.043.8665731
TCAACGC25200.040.660664
ATCAACG25100.040.6369483
CAACGCA26750.038.3046235
AACGCAG27800.037.026946
TACATGG54350.032.258512
GTACATG54100.032.2071341
TATCAAC32300.032.160652
ACATGGG56000.030.3847453
TTAGGTA6400.030.1108064
TAGGTAT5950.030.0182345
ACGCAGA34600.029.7476987
GTCTTAG7750.029.1666931
AGGTATA6300.028.3505526
CGCAGAG37600.027.374218
GGTATAG6750.027.1547747
ACCTAAG10100.027.0429631
CTTAGGT6800.026.2669563
GTACTAG909.410296E-426.1622521
Produced by FastQC (version 0.11.2)