FastQCFastQC Report
Sun 14 Apr 2019
SRR6575825_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575825_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1192560
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA27180.227913060978064No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22180.18598644931911182No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC19290.16175286778023748No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14100.11823304487824512No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13510.11328570470248876No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13310.11160864023613067No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13150.11026698866304421No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11950.10020460186489569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18350.056.602231
TCAACGC22250.046.250734
ATCAACG22600.045.95033
CAACGCA23750.043.7253385
AACGCAG24350.042.647916
GGTATCA5850.039.3656581
TATCAAC28600.036.3195082
ACGCAGA30150.034.287827
CTTAGGT5600.033.564213
CGCAGAG31750.032.7079288
TAGGTAT5500.032.4657485
GTACATG46050.031.4339081
TTAGGTA5900.031.0611174
GTCTACG3050.030.8182141
AGGTATA5800.030.7864846
TACATGG47300.030.5064032
ACATGGG48750.029.5915853
TACGTGT3250.028.9168594
GTCTTAG6850.028.8161561
GCAGAGT36700.028.2939959