FastQCFastQC Report
Sun 14 Apr 2019
SRR6575825_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575825_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1192560
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA29610.24828939424431473No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23100.19370094586435904No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC19170.16074662910042262No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14650.12284497216072986No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13070.10959616287650098No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12790.10724827262359965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17650.059.495211
ATCAACG23650.043.916353
TCAACGC23900.043.6536144
CAACGCA24350.042.653875
AACGCAG25200.041.2134176
GGTATCA6000.038.456231
TATCAAC30400.034.3240972
ACGCAGA30900.033.6095357
GTACATG47250.031.891141
TTAGGTA6550.031.5701394
TACATGG48100.030.9766122
CTTAGGT6850.030.8735873
TAGGTAT6500.030.3669415
CGCAGAG34600.030.0368618
ACATGGG50250.029.7410433
GTCTTAG8450.029.5352761
AGGTATA6700.029.4592296
ACCTAAG9900.029.0145971
GGTATAG6900.028.604147
TATAGTA7200.028.0849959
Produced by FastQC (version 0.11.2)