FastQCFastQC Report
Sun 14 Apr 2019
SRR6575826_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575826_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198964
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5460.2744215033875475No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3790.19048672121589835No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3600.18093725498080054No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3410.17138778874570276No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2980.14977583884521825No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2940.14776542490098712No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2550.12816388894473374No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2540.12766128545867594No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2310.11610140527934702No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2310.11610140527934702No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2190.11007016344665367No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2120.10655193904424921No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2100.10554673207213365No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2050.10303371464184476No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2040.10253111115578697No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAT250.00523551156.3841675
GCTAACC451.6117652E-552.207563
GTATAGT503.0053743E-546.9868058
GTCTTAG708.579627E-846.9868051
CTAATTC405.673188E-446.9868053
GTATCAA6450.045.8940931
TATAGTA555.2760304E-542.7152759
TTAGGTA450.001013331941.766054
GTACATG11950.040.4990841
TACATGG12200.039.6691862
GGGAATA952.1071173E-839.5678378
AACGCAG7550.039.2075356
CTAACCT608.812352E-539.1556744
ACATGGG12350.038.806923
AGGTATA500.001700991237.5894476
GGTATAG500.001700991237.5894477
GTACTAC500.001700991237.5894476
GGGTACC2400.037.197897
TAGGTAT550.00271537234.1722225
AATACTG550.00271537234.1722226