FastQCFastQC Report
Sun 14 Apr 2019
SRR6575826_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575826_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198964
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5840.2935204358577431No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4270.21461168854667179No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3260.16384873645483605No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3240.1628435294827205No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3020.15178625278944935No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2840.1427393900404093No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2830.14223678655435154No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.13972376912406265No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2540.12766128545867594No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2210.11107537041876922No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2140.10755714601636476No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2110.10604933555819145No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2000.10052069721155586No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAT450.001006429541.824081
ACCGAAG450.001008925541.8030438
GTATCAA7150.040.142341
CCGTCGT550.002703606834.2024889
ATAATTA550.00271362834.17674
ATAGTCA550.00271362834.17674
GTACATG12400.033.3918041
TACATGG12650.032.6907542
AACGCAG8450.032.2555276
TATTATC753.2687583E-431.3286422
ACATGGG13200.030.6166293
CATGGGT3250.030.3646834
TGGGAGT1252.3496796E-730.0754956
CCTTAAA804.736014E-429.4075551
TCAACGC9500.029.1851044
GAATAGG650.006108945228.955131
GAATACT650.006146604728.9187474
ATCAACG9650.028.731453
CAACGCA9650.028.2444745
ATATGAG350.0088032126.87338368-69
Produced by FastQC (version 0.11.2)