FastQCFastQC Report
Sun 14 Apr 2019
SRR6575827_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575827_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1560577
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA19530.12514601970937672No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA18820.12059642042654736No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18580.11905852771122476No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18560.11893036998494788No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA18030.11553419023861047No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA17470.11194577390285772No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17140.10983117141928915No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16680.10688354371492083No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16370.10489709895762914No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15610.10002710535910757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26900.055.746091
ATCAACG34100.043.415893
TCAACGC34050.043.3416144
CAACGCA36300.040.7846265
AACGCAG37100.040.158546
AGGTATA4400.036.3177386
GGTATCA10600.034.1476551
TATCAAC44800.033.1577532
ACGCAGA45800.032.4275477
TAGGTAT4950.032.2824335
GTACATG65950.030.5786631
TACATGG66650.030.257512
CGCAGAG49500.030.0036728
ACATGGG68900.029.059153
GGTAATC3400.029.0290558
TTAGGTA5500.028.1996544
GGTATAG5500.028.1996547
CTTAGGT5550.027.9456043
GCAGAGT53950.027.615979
GTCTTAG7700.027.4724771