FastQCFastQC Report
Sun 14 Apr 2019
SRR6575828_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575828_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1426721
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA21370.14978401523493381No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16990.11908424982880325No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16770.11754225247963687No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16340.11452834856990259No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15860.11116399071717596No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15730.11025281046539583No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14640.10261291450816241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22450.056.5960581
ATCAACG30250.041.1685183
TCAACGC30150.041.1491974
GGTATCA8550.038.527491
CAACGCA32250.038.469715
AACGCAG33200.037.5104756
TATCAAC39250.031.8483352
ACGCAGA40550.030.7103357
GTACATG57050.029.2827221
GTCTTAG7100.028.5002631
TACATGG58800.028.2125342
CGCAGAG44600.027.8347858
ACATGGG59650.027.3378123
GCAGAGT48550.025.6670199
GGTACCT9250.024.40168
CTAAGAC10400.023.9489923
AGAGTAC41300.023.5688910-11
TATACAG3600.023.4971285
GGGTACC9700.023.2540727
ACCTAAG7950.023.0853391
Produced by FastQC (version 0.11.2)