FastQCFastQC Report
Sun 14 Apr 2019
SRR6575829_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575829_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267361
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14830.11701480477938014No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14030.11070247545884716No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13550.10691507786652737No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12810.10107617324503436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16500.057.9843061
TCAACGC22900.040.6375054
ATCAACG23150.040.4016763
CAACGCA23450.040.2856645
AACGCAG24350.038.0245936
GGTATCA5900.034.349061
TATCAAC28900.032.525912
GTACATG45650.030.1467461
ACGCAGA30800.029.755287
TACATGG45600.029.6841742
ACATGGG47550.028.0714683
CGCAGAG33300.027.6625378
GCAGAGT38250.024.4512929
AGAGTAC31200.024.10158310-11
GTCTTAG4700.023.0636671
AGGTATA2653.6379788E-1123.0565766
GCTATAG1852.7411625E-722.9281581
CATGGGG29600.022.3884834
TAGGTAT2555.6024874E-1022.1176195
GTATAAA3101.2732926E-1121.2845631
Produced by FastQC (version 0.11.2)