FastQCFastQC Report
Sun 14 Apr 2019
SRR6575831_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575831_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1622119
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16850.10387647268788541No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16410.10116397132392876No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16340.10073243701602658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21500.054.6603361
ATCAACG27900.041.4388773
TCAACGC27900.041.2704284
GGTATCA8600.039.3554461
CAACGCA29400.039.164795
AACGCAG31350.036.7287036
TATCAAC37850.030.8004132
GTACATG59650.030.0251581
TACATGG60100.029.487482
ACGCAGA39050.029.4864237
ACATGGG61200.028.7976423
CGCAGAG42150.027.3177918
AGGTATA5200.027.1140886
GTCTTAG7850.025.7495461
TAGGTAT5550.025.4041885
ACCTAAG6300.024.623181
TTAGGTA5750.024.5205654
GCAGAGT47450.024.3655349
CTTAGGT5300.023.942253
CTAAGAC10000.023.4988733
Produced by FastQC (version 0.11.2)