FastQCFastQC Report
Sun 14 Apr 2019
SRR6575831_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575831_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1622119
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16600.10233527873109187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22000.051.8630371
ATCAACG28100.039.9799923
TCAACGC28450.039.3229264
CAACGCA29500.038.0814635
AACGCAG30900.036.2028546
GGTATCA9700.032.080231
TATCAAC36050.031.2937152
GTACATG58250.030.1910461
ACGCAGA37850.029.5516577
TACATGG59050.029.5328372
ACATGGG60950.028.380843
CGCAGAG41150.027.1826178
GTCTTAG6200.026.6159271
ACCTAAG7450.025.3144781
TAAGACA11300.024.1268524
CTAAGAC11000.023.502883
GCAGAGT48550.023.039449
CATGGGG38250.022.1203614
TACGTGT3650.021.8930974
AGAGTAC40250.021.77695510-11
Produced by FastQC (version 0.11.2)