FastQCFastQC Report
Sun 14 Apr 2019
SRR6575832_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575832_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450920
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15520.10696661428610812No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15420.1062773964105533No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14970.10317591597055661No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14800.10200424558211342No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14720.10145287128166956No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14680.10117718413144763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20650.052.814761
TCAACGC26550.040.3504264
ATCAACG27750.038.6055453
CAACGCA28250.037.922265
AACGCAG29100.036.8145646
GGTATCA9000.034.4737741
TATCAAC34800.030.9324022
GTACATG55650.030.6640011
TACATGG56050.030.0237452
ACGCAGA36150.029.7649367
ACATGGG58850.028.5035883
CGCAGAG39500.027.1216148
TAGGTAT4100.026.3585645
TTAGGTA5050.025.1217654
CTAAGAC8900.024.2854213
GTCTTAG7100.023.8358971
GCAGAGT46250.023.56979
TAAGACA9400.023.4935054
CATGGGG36150.023.266044
CTTAGGT4700.022.9936433
Produced by FastQC (version 0.11.2)