FastQCFastQC Report
Sun 14 Apr 2019
SRR6575832_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575832_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450920
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA15760.1086207371874397No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15700.1082072064621068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19600.049.0372771
ATCAACG23550.039.7077673
TCAACGC23950.039.2407954
AGGTATA3200.038.1800656
GTACTAA1003.257628E-837.69141
CAACGCA25100.037.442915
AACGCAG26250.036.160586
TAGGTAT3550.035.739525
GGTATCA8800.033.729521
TTAGGTA4200.033.5648964
CTTAGGT4200.032.4460643
GTCTTAG5700.032.236061
TATCAAC30900.030.4179362
GTATACG953.6912774E-529.7563651
GGTATAG4500.029.2397587
GTACATG52000.028.6309641
ACGCAGA32950.028.5235027
TACATGG51700.028.0883452
ACATGGG53550.027.0274163
ACCTAAG6000.026.6980741
Produced by FastQC (version 0.11.2)