FastQCFastQC Report
Sun 14 Apr 2019
SRR6575835_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575835_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239205
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC48960.3950920146384174No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA48150.3885555658668259No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC40170.32415944093188775No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG22810.1840696252839522No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT22460.18124523383943736No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG22070.17809805480126373No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG21470.17325624089638114No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC20120.16236215961039538No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT19080.15396968217526558No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA15610.12596785842536143No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC15490.12499949564438492No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA13670.1103126601329078No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA13480.10877941906302831No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT13280.10716548109473412No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT12620.1018394857993633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27800.048.7103651
TCAACGC33700.039.188384
ATCAACG34000.038.7043723
AAGGGTA3700.038.106625
CAACGCA35200.037.7854585
GGTATCA10300.036.976131
AGGGTAA4000.036.423586
AACGCAG36900.036.0446626
TATCAAC37450.035.4055182
ACCAGAT16400.033.81575494
ACCTAAG16100.032.416851
GTACATG62500.031.897711
GGTAATC4600.031.6726748
GTCTTAG13050.031.346021
CTAAGAC17500.030.8845083
ACATGGG64850.030.5832313
GTGCAAG6050.030.309791
TAAGACA19100.030.2658374
CCTAAGA18350.029.9794392
ACGCAGA44550.029.8551757
Produced by FastQC (version 0.11.2)