FastQCFastQC Report
Sun 14 Apr 2019
SRR6575835_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575835_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239205
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA53990.43568255454101623No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC48180.38879765656207005No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC38650.31189351237285196No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG21900.17672620752821366No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG20310.16389540068027483No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT20020.1615551906262483No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG18220.1470297489116006No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT18110.14614208302903878No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16020.1292764312603645No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC15790.1274204025968262No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA14840.1197541972474288No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT13380.1079724500788812No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA12930.10434108965021928No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT12830.10353412066607219No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA12700.10248506098668098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25350.050.9211431
ATCAACG32000.040.101043
TCAACGC32200.039.9979444
CAACGCA32500.039.4825065
AACGCAG34000.038.1553656
GGTATCA9400.037.5888441
TATCAAC36750.035.173722
AAGGGTA3350.035.0768555
GTCTTAG13300.035.067851
AGGTATA11600.034.441846
GGTAATC3600.033.9424938
CTTAGGT12050.033.547053
TTAGGTA12250.033.383064
TAGGTAT12100.033.0186275
ACCAGAT18200.032.53921594
GTACATG59700.032.1177861
GGTATAG12850.031.0877087
ACCTAAG18100.030.4531921
ACGCAGA42350.030.406757
ACATGGG62400.030.2066653
Produced by FastQC (version 0.11.2)