FastQCFastQC Report
Sun 14 Apr 2019
SRR6575836_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575836_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1099883
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA44630.4057704319459433No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC43340.3940419117306113No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC37800.34367291793763516No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG20810.18920194238841767No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT20070.18247395404783964No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG19440.17674607208221238No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG18050.16410836425328876No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC18000.16365377044649296No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT16710.15192525023116094No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC14190.12901372236865194No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA14060.12783177847098284No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA12850.11683060834652413No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA11950.10864791982419948No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT11910.10828424477876282No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA11450.10410198175624134No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG11430.10392014423352303No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT11350.10319279414264973No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA11040.10037431254051568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25650.049.4729921
GGTATCA9950.042.5119671
ATCAACG30550.040.607433
TCAACGC30750.040.496134
CAACGCA31500.039.382765
AACGCAG32600.038.0538946
TAGACGC500.001703633937.5926364
AGGTATA12150.037.1285256
TATCAAC33950.036.5506742
TAGGTAT12850.035.471655
GTCTTAG14050.035.1240651
CTTAGGT12900.034.605623
TTAGGTA13050.034.2078554
GGTATAG13300.033.9181637
ACCAGAT15800.032.11736794
GTACATG55450.031.1916281
ACGCAGA40050.030.9752047
TCTTAGG16150.029.9775372
CGCAGAG41550.029.9700628
ACATGGG57050.029.8171223
Produced by FastQC (version 0.11.2)