FastQCFastQC Report
Sun 14 Apr 2019
SRR6575839_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575839_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933823
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA18230.19521900831313857No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15630.1673764728433547No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC13150.1408189774721762No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11830.1266835363875167No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10710.11468982880053287No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10280.11008510178053015No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10190.1091213217065761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5000.053.5767061
GTATCAA12750.052.7104841
TCAACGC17100.038.468924
ATCAACG17350.037.9146163
CAACGCA18300.035.9463735
AACGCAG18950.034.9613346
GTACATG33450.032.1744041
CGTTACA600.00416183831.3314062
TACATGG34400.031.0126272
ACATGGG34550.030.1913833
TAAGACA9900.029.9008454
ACCTAAG8550.029.6823841
TATCAAC22900.029.5527672
CTAAGAC9400.028.9920063
CGTAGTG650.006155433628.9212972
ACGCAGA23550.028.132377
ATGGGAG8650.027.7033425
CCTAAGA9550.027.5585142
CGCAGAG26200.025.286928
GCAGAGT27750.024.2131429
Produced by FastQC (version 0.11.2)