FastQCFastQC Report
Sun 14 Apr 2019
SRR6575839_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575839_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933823
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA19680.21074657617128728No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16730.17915600708057094No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12000.12850400986054103No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11020.11800951572193018No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10330.11062053515494906No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10090.10805045495773824No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10010.10719376155866797No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9710.10398116131215444No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9700.10387407463727065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12450.053.313661
GGTATCA4700.042.0668681
TCAACGC16200.039.7419744
ATCAACG16650.038.667873
CAACGCA17150.037.5405275
AACGCAG18200.035.3747256
GTACTAA801.1458571E-535.3061181
GTACATG31150.033.247071
TACATGG32650.031.9548632
ACATGGG33250.030.9525473
CTTAGGT5050.030.7090573
AGGTATA4900.029.7310036
CTAGACG953.75471E-529.6805253
TAGGTAT4950.029.4306875
TATCAAC22250.029.3597242
TTAGGTA5050.028.8479024
ACGCAGA22550.028.550787
GTCTTAG5550.027.9904381
GTATAGT5300.027.50638
GGTATAG5300.027.4871527