FastQCFastQC Report
Sun 14 Apr 2019
SRR6575840_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575840_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740720
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC16390.22127119559347663No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA14040.18954530726860352No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12760.1722648234150556No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT12330.16645966087050437No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC12100.16335457392806998No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10250.13837887460848905No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA10200.13770385570795984No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG9750.13162868560319688No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT9570.12919861756129172No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA9090.12271843611621125No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8750.11812830759261259No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8390.11326817150880224No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA8190.11056809590668538No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7940.10719300140403933No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7910.10678799006372179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTTA1251.5097612E-1037.599627
ATTGTAC757.4405307E-637.599623
GTCTATA851.7524437E-533.180621
CTTACTC600.00416023931.333023
GGGTACC3200.029.3747027
TGGGTAC3400.029.029126
ATGGGTA3650.028.3284825
CATGGGG20100.028.293254
TAATAAT3050.027.7374253
TCAAGAC2253.6379788E-1227.1552813
GTATAAG2850.026.3892631
ATGGGGG12850.026.3343665
GGTACCT3600.026.1108478
ATAGGTA4000.025.84974194
GTATCAA29250.025.551911
ACGTTAA1851.002627E-825.4051518
GTACTAG5250.025.06981
TAGTACG950.001304224424.7365934
TTATGTA1351.4551228E-524.3734152
GTTAATA3700.024.1381511
Produced by FastQC (version 0.11.2)