FastQCFastQC Report
Sun 14 Apr 2019
SRR6575841_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575841_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684026
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA14270.20861780107773684No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC13860.20262387687017747No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13230.19341370064880575No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11150.16300549979094361No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC9880.1444389540748451No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA9220.13479019803340808No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8160.11929371105776682No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC7890.11534649267717895No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA7870.11505410613046872No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA7650.11183785411665638No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT7470.10920637519626446No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7220.10555154336238681No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC7150.10452819044890108No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG7000.10233529134857447No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7000.10233529134857447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG3950.039.2941551
CATGGGG18150.031.3284364
CTTAGGT1351.3274985E-831.3284363
CGCCTAC2450.030.6890817
ATGGGGG10450.029.6795715
TAGGCAT5750.029.4214885
TCTAACG2400.029.3704092
TATTAAG804.783258E-429.3704092
TATATAC650.006157148628.9185563
TAGTACT5600.028.5312544
CTAACGC2700.027.8474983
GTCCTAC3850.026.876521
CTGTGCG5950.026.872599
TCTTAGG2450.026.8529452
CTAGACG700.00884030226.8529433
ATGGGCG700.00884030226.8529435
CTAGGCA6700.026.652554
GTACTGT6100.026.1926256
CCTAGAC1101.01882746E-425.6323572
TACTGTG7000.025.5102987
Produced by FastQC (version 0.11.2)