FastQCFastQC Report
Sun 14 Apr 2019
SRR6575842_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575842_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988246
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA40230.4070848756281331No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC31140.3151037292334095No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC27280.27604462856414297No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA19140.1936764732667777No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC17440.17647427867150486No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT17090.1729326503724781No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC15500.1568435389568994No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC14790.14965909297887367No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG13390.13549257978276663No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG13360.13518901164285008No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT13280.13437949660307252No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT12870.1302307320242126No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG12730.1288140807046019No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT11640.11778443828763284No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA10840.10968928788985738No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC10470.10594528083088624No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT9900.10017748617247121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAATC3550.050.3090258
AAGGGTA3800.049.472865
GTGCAAG3350.047.712771
AGGGTAA4200.043.6421286
TAGGCGG450.001013969841.7770845
TAGGTAT8900.035.909515
AGGTATA8700.035.654586
TTAGGTA8750.035.4508364
GTACTTG4300.034.9850121
CTTAGGT8800.034.715333
GTAATCA5300.033.697559
TGCAAGG5050.032.5819552
ACCTAAG15650.031.2406081
TCTTAGG10200.030.8798472
GTATAAG4300.030.6118851
GTACTGT7800.029.5251486
TAGTACT6850.029.503164
GGTATAG10600.029.2636627
CAAGGGT5850.028.9225944
GGGTACC2950.028.6774887
Produced by FastQC (version 0.11.2)