FastQCFastQC Report
Sun 14 Apr 2019
SRR6575842_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575842_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988246
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA43560.4407809391588734No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC31800.32178222831157427No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC26380.26693758436664555No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA19150.1937776626467499No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT17490.17698022557136583No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC16310.16503987873464704No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG14020.1418675107210148No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC13690.13852826118193245No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT12830.12982597450432382No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12500.12648672496524144No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA11820.1196058471271323No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11590.11727849138777188No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG11470.11606421882810555No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG11070.11201664362921783No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT11000.11130831796941247No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC10340.10462981889124773No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA9910.10027867555244341No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT9910.10027867555244341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT6250.035.3434684
GGTAATC2700.033.0719038
ACCTAAG15150.032.6514171
AGGTATA7800.032.5363246
AAGGGTA3050.032.3585325
TAGGTAT8000.032.310385
TTAGGTA7650.031.9472314
CTTAGGT7750.031.535013
GTGCAAG3300.031.4075551
GTACTGT7450.030.2798886
CCTAAGA17100.029.6837642
CGTTAAC1751.6734703E-1029.6128371
CTGTGCG7250.029.1704989
TAAGACA18200.028.9226424
CTAAGAC17250.028.8807243
TCTTAGG9400.028.4995732
CATGGGG32200.028.3163434
GTATCAA38700.028.242451
CTAGTAC7750.027.8963553
GTCCTAA1554.9665687E-827.3549651
Produced by FastQC (version 0.11.2)