FastQCFastQC Report
Sun 14 Apr 2019
SRR6575843_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575843_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922355
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA42420.45990968770158996No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC29490.31972505163413223No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC25200.27321367586233064No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20680.22420868320765863No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT18240.19775466062416314No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC15450.1675060036536908No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC13340.14462977920648776No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG13300.14419610670511895No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12530.13584791105376998No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11570.1254397710209193No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT11470.12435558976749733No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11440.12403033539147075No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG10950.1187178472497032No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA10370.11242959597985591No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC9860.10690027158740398No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG9720.10538241783261326No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT9670.10484032720590229No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA9290.1007204384428989No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA9260.10039518406687228No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG450.00101436441.7734875
AGGTATA8650.039.117376
TAGGTAT8900.038.0185665
TTAGGTA8900.037.4905324
GTGCAAG3400.037.3744051
CTTAGGT8950.037.2810863
GGTAATC2950.036.662178
TCTTAGG10450.034.62992
AAGGGTA3200.033.7777825
GTACTAG5050.032.6186561
AGGGTAA3350.032.2653436
AACCGTG2150.030.599857
ACCTAAG16100.030.1093081
GGTATAG11600.029.167847
GTACTTG3550.029.1664621
GACGTAC650.006112729728.96251
GTATAGT11800.028.6921338
TGCAAGG4100.028.6571482
TGAACCG2300.028.6057595
CTAAGAC17550.028.116773
Produced by FastQC (version 0.11.2)