FastQCFastQC Report
Sun 14 Apr 2019
SRR6575844_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575844_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1356617
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC29060.21420931626243814No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA28370.20912313497472018No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC22250.16401091833583098No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15870.11698216961751179No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15540.1145496481320815No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15160.11174856278522237No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14620.10776807308179095No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14470.10666238149750445No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14410.10622010486378987No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13680.10083907248692887No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13650.10061793417007157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23800.050.7665521
GGTATCA9400.045.0128141
TCAACGC28650.041.666454
ATCAACG29600.040.1704063
CAACGCA30250.039.92875
AACGCAG30850.038.9997836
TATCAAC37600.032.1341482
GTACATG54550.031.8019051
GGTAATC3550.031.7731518
TACATGG55400.031.3139692
ACGCAGA38550.031.2099467
ACCTAAG10750.030.1760311
ACATGGG57350.029.6655623
CGCAGAG40850.029.3376668
GATACGT650.00615596928.9217154
CTAAGAC12750.028.382983
GCAGAGT44200.027.2204369
GGGTACC8300.027.1794437
GGTACCT8400.026.8558798
TAAGACA14100.026.6654134