FastQCFastQC Report
Sun 14 Apr 2019
SRR6575844_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575844_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1356617
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA31130.22946786012559184No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC28230.20809115616271948No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC22900.1688022485344058No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15620.11513935031036762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21100.055.6809351
TCAACGC27300.043.0442244
ATCAACG27550.042.483013
CAACGCA28350.041.7800565
AACGCAG29300.040.1045766
GGTATCA8050.038.0984271
TATCAAC35500.032.969212
GTACATG53100.032.7884941
ACGCAGA37400.031.289697
TACATGG55100.031.2224542
ACATGGG56850.030.261343
GTCTTAG10100.029.8984171
TTAGGTA9350.029.1577454
ACCTAAG12950.029.1480711
CTAAGAC14600.028.975253
TAGGTAT9250.028.9637415
CGCAGAG41100.028.4728558
AGGTATA9500.028.2015386
CTTAGGT9700.028.1056633
GGTATAG9850.027.1964427
Produced by FastQC (version 0.11.2)