FastQCFastQC Report
Sun 14 Apr 2019
SRR6575845_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575845_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205942
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA27140.22505228277976885No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC26640.22090614639841719No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC21980.18226415532421958No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15610.12944237782579926No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14410.11949165051055523No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13760.11410167321479806No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13580.11260906411751145No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13520.11211152775174925No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13240.1097896913781923No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12570.10423386862718108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20600.052.7166521
TCAACGC24750.042.716364
ATCAACG25200.041.9535683
GGTATCA7550.041.7187161
CAACGCA25750.041.0574725
AACGCAG27150.039.4595346
GGTAATC3150.032.815658
GTACATG48650.032.661561
TACATGG49250.032.2623142
TTAGGTA6700.032.2604184
TATCAAC33000.031.9094352
TAGGTAT6900.031.3253295
ACGCAGA34200.031.1866477
GTCTTAG8200.030.958721
ACATGGG51650.030.2942923
CGATAGG804.7742427E-429.3821181
CGCAGAG36400.029.301748
CTTAGGT7250.029.1649653
AGGGTAA3450.028.6013896
AGGTATA7600.028.4401046