FastQCFastQC Report
Sun 14 Apr 2019
SRR6575845_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575845_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205942
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA29870.24769018742194898No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC27930.2316031782623045No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC21190.17571325984168393No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14550.1206525686973337No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12420.10299002771277557No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12160.10083403679447271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18000.053.9002341
GGTATCA7050.045.4271741
TCAACGC22750.041.931174
ATCAACG23100.041.2958563
CAACGCA23900.040.110185
AACGCAG23950.040.0264436
GTACATG47650.033.2102621
TACATGG48350.032.558972
TATCAAC29750.032.222972
ACATGGG49600.031.8331763
ACGCAGA31600.030.4864717
ATTATCC13600.028.6788393
CGCAGAG33550.028.5922458
TTATCCT15950.027.1050074
CATGGGG30100.026.6963584
TATTATC15150.026.0548782
GCAGAGT37000.025.926219
TATCCTG15800.025.2804155
AGGTATA7350.024.9344436
TAGGTAT7550.024.8963345
Produced by FastQC (version 0.11.2)