FastQCFastQC Report
Sun 14 Apr 2019
SRR6575848_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575848_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341108
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4640.1360272992717849No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4600.1348546501401316No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4580.1342683255743049No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGA301.3715621E-462.650256
GTATCAA14450.036.094351
AACGCAG17500.030.340626
TACATGG23900.029.686782
TGAACCG804.781248E-429.3673045
ATCAACG17800.029.301313
CAACGCA18000.029.2367845
GTACATG24350.029.1381551
ACATGGG24500.028.5761853
TCAACGC18450.028.5236914
ACGCAGA19350.027.1970087
ATGGGAG4700.026.9929285
TATCAAC19350.026.9541762
ACCGATG700.00883875526.8501078
GTACTAT700.00883875526.8501071
CTAGCGT350.00884484226.85010792-93
CGCAGAG19700.026.4752948
GGTACCT2357.2759576E-1225.9931898
ATGGGTA3100.025.767445
GTATATG1659.3010385E-825.6296481
Produced by FastQC (version 0.11.2)