FastQCFastQC Report
Sun 14 Apr 2019
SRR6575848_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575848_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341108
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5440.15948028190485125No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4200.12312815882359839No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4200.12312815882359839No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4040.11843756229698511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14550.035.558491
GTACATG24850.031.797761
TACATGG24700.031.772472
ACATGGG24600.031.5195733
GTTATAT753.2568316E-431.3561231
AACGCAG17050.029.766716
ATCAACG17400.029.7081013
TCAACGC17550.029.1864224
CAACGCA17800.028.77655
CCGTGCT856.783135E-427.6631099
TATCAAC19200.026.9229662
CATGGGG9000.026.6292634
ACGCAGA19100.026.5718547
GGGTACC2305.456968E-1226.5611577
CGCAGAG19350.026.2477878
CATGGGT5950.026.0632444
CATGGGA14050.024.4161974
ATGGGTA2751.8189894E-1223.9236185
CTTAAAC1000.001757718223.4964083
GCAGAGT21650.023.2421239
Produced by FastQC (version 0.11.2)